20 credits at level HE5
This module is a combination of three topics namely biochemistry, genetics and microbiology. They are presented as three consecutive units but all contain elements that inter relate.
These theoretical aspects are supported by the module 'Practical Skills in Biomolecular Science'
i) To develop an understanding of key biochemical principles, common to most living organisms
ii) To develop an understanding of the biochemical process of selected metabolisms
iii) To illustrate how specialised biological functions/malfunctions can be explained by a knowledge of biochemical pathways
i) To investigate in depth the chemical nature of DNA and its recent categorisation
ii) To discuss how genes function and interact
iii) To understand how the possible 'end product' of genetic processes can be altered by environmental factors
iv) To develop an awareness and knowledge of how DNA technology can be use to benefit mankind in the field of agriculture, medicine and forensic science
v) To discuss safety and ethical issues of gene manipulation
C) Microorganisms and Man
i) To introduce the theory of practical methods in microbiology
ii) To understand how certain microbes can be pathogens
iii) To investigate how some microorganisms can be exploited to benefit mankind.
Biochemical reactions - key principles, comparison of chemical and biochemical reactions. Energy requirements. The importance of cell organisation into pathways. Regulation. Biochemical dependency of organisms on each other
An introduction to thermodynamics. First and second laws of thermodynamics. Biochemical systems and work types i.e. mechanical, electrical and synthetic.
Biological Importance of Water Properties of water. Electrostatic forces. Van der Waals forces (attractive/repulsive). Solubility of small molecules, pH, buffed solutions e.g. buffering of blood plasma - carbon dioxide-carbonic acid - bicarbonate system.
Proteins - The Structural and Functional Molecules of Life. Amino acid content of selected proteins. Theory of high - pressure liquid chromatography and how used in quantitative amino acid analysis.
Three dimensional Structure of Proteins. The Ramachandran plot, Prions disease. Molecular mass of selected proteins.
Functional Diversity of Proteins. Ten major groupings of proteins found in living organisms e.g. proteins of cytoskeleton, plasma membrane proteins. Evolution of proteins e.g. cytochrome C.
Characterisation and Purification of Proteins. Salting in, salting out. Gel exclusion chromatography. Ion-exchange chromatography. Electrophoresis. Ultra-centrifugation. Protein assays.
Enzymes - as key functional proteins. Enzyme terminology - six classes of enzymes. Enzyme Kinetics. Michaelis Constant - effects of temperature and pH on enzyme activity. Enzyme inhibition - competitive/non-competitive/un-competitive.
Biochemistry of Disease and Defence. Cholesterol metabolism and heart disease. Carbohydrates and cell recognition. Cancer and carcinogensis
The Nature of DNA. Chemistry and categorisation of DNA (including coding, non-coding, extragenic, satellite). Introns, exons pseudogenes. Ancient DNA.
Genetic Interactions. Codominance, epistasis, polygenic inheritance. Pleiotropism e.g. sickle cell condition. Effects of the environment on gene expression such as influence of temperature and nutrients (example lac operon). Role of structural, regulatory, operator and promoter genes
Genetic Engineering. Technique of gene manipulation. Detailed account of role of different restriction enzymes. Use of plasmid vectors e.g. pBR 322. Restriction maps. RFLP analysis. Applications of DNA technology, Human Genome Project, uses in medicine and forensic science, agricultural benefits. Safety and ethical issues.
Microorganisms and Man
Pure culture technique. Sterilization. Microbial nutrition. The use of selective media. Identification. Classification.
Microbial pathogenesis. Human pathogens.
Traditional microbial processess (using yeast). Microbial production of chemotheapeutic agents. Production of useful products using recombinant DNA technology.
Lectures and tutorials.
Class contact (13 x 3 hours) = 39 hours
Private study = 120 hours
3 hour exam = 3 hours
2 coursework @ 19 hours = 38 hours
Total = 200 hours
when you have successfully completed this module you will:
to demonstrate that you have achieved the learning outcome you will:
|1.||Demonstrate a detailed knowledge and understanding of biochemistry, genetics and micro biology||Explain key biochemical, and genetic principles and processes. Explain the possible impact of micro organisms on mankind|
|2.||Be able to express yourself clearly in writing and have developed your capacity to be analytical and critical.||Produce clear, concise, reasoned essays with substantial critical and analytical content|
|3.||Be able to effectively use a wide range of reference sources.||Approriately select, evaluate and incorporate up-to-date reference material into your work.|
Your achievement of the learning outcomes for this module will be tested as follows:
|Description||2 essays 2,500 words each||Unseen examination|
Before taking this module you must have successfully completed the following:
No restrictions apply.
Alberts, B. et al. (2002) Molecular Biology of the Cell. (4th Ed) Garland
Andrews, A.T. (1986) Electrophoresis. Theory, Techniques and Biochemical and Clinical Applications. (2nd Ed) Oxford University Press (out of print, but available from LS and D)
Berg, J.M., Tymoczko, J.L. & Stryer, L. (1999) Biochemistry (5th Ed). W.H. Freeman & Co.
Brown, T.A. (1998) Genetics: A Molecular Approach (3rd Ed) Thornes
Campbell, N.A. & Reece, J.B. (2001) Biology (6th Ed). Benjamin Cummings
Connor, J.M. & Ferguson-Smith, M.A. (1997) Essential Medical Genetics (5th Ed). Blackwell Scientific Publications
Griffiths A.J.F.,et al. (2000) An Introduction to Genetic Analysis (7th Ed). Freeman
Holme, D.J. & Peck H. (1998) Analytical Biochemistry (3rd Ed). Prentice Hall
Jones, A., Reed, R. & Weyers, J. (2003) Practical Skills in Biology (3rd Ed). Prentice Hall
Kirby, L.T. (1990) DNA Fingerprinting. Stockton Press Macmillan Publishers
Matthews, C.K. , Van Holde, K.E. & Ahern, K.G. (2000) Biochemistry (3rd Ed). Benjamin/Cummings
Reed, R., et al. (2003) Practical Skills in Biomolecular Sciences (2nd Ed). Pearson/ Prentice Hall
Russell, P.J. (1998) Genetics (5th Ed). Longman
Snustad, P.D., Simmons, M.J., & Jenkins, J.B. (2003) Principles of Genetics (3rd Ed). John Wiley & Sons
Strachan, T. (1992) The Human Genome. BIOS Scientific Publishers (out of print, but available from LS and D)
Stanier, R.Y., Ingraham, J.L. & Wheelis, M.L. (1995). General Microbiology (5th ed). Macmillan.
Strickberger, M.W. (1990) Genetics (3rd Ed). Maxwell Macmillan (out of print, but available from LS and D)
Williams, J.G & Patient, R.K. (1989) Genetic Engineering. IRL Press
Zubay, G.L. (1998) Biochemistry (4th Ed). Wm. C. Brown Publishers
|Host Subject Group:||Psychology and Life Sciences|
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